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siRNA Id: | aspsirna2426
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siRNA Name: | 21
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| Gene: | K-RAS (KRAS) |
| Disease: | NA |
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Wild allele/target (5'->3'): | AACUCCUCCGCGCAACUUU
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Mutant allele/target (5'->3'): | AACUCCUCCGCGAAACUUU
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Target Mutation: | NA
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Wild siRNA (As strand): | UUGAGGAGGCGCGUUGAAA
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ASP-siRNA (As strand): | UUGAGGAGGCGCUUUGAAA
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Mismatch position in siRNA: | U7G |
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Mutant allele (5'->3'): | AACUCCUCCGCGAAACUUU
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ASP-siRNA (As strand): | UUGAGGAGGCGCUUUGAAA
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ASP-siRNA efficacy for mutant allele: | 7
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Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
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ASP-siRNA (As strand): | UUGAGGAGGCGCUUUGAAA
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ASP-siRNA efficacy for wild allele: | 70
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Percentage discrimination for ASP-siRNA: | -63
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Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
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Wild siRNA (As strand): | UUGAGGAGGCGCGUUGAAA
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Wild sirna efficacy for wild allele: | 70
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Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
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ASP-siRNA (As strand): | UUGAGGAGGCGCUUUGAAA
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Wild sirna efficacy for mutant allele: | 50
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Percentage discrimination for wild siRNA: | 20
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| Concentration: | 100nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P01116 |
| Target sequence: | GAGGATGCTTTTTATACATTGGTGAGGGAGATCCGACAATAC |
| Genbank Accesion: | NM_033360.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 190070 |
| Reference: | US007576197B2 |
