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siRNA Id: | aspsirna2976
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siRNA Name: | siSNCA_T1-5(10A)
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| Gene: | Synuclein, alpha (SNCA) |
| Disease: | Parkinsons disease (PD) |
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Wild allele/target (5'->3'): | UUCUAAAAGUCACUAGUAG
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Mutant allele/target (5'->3'): | UUCUAAAAGACACUAGUAG
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Target Mutation: | SNCA locus triplication
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Wild siRNA (As strand): | AAGAUUUUCAGUGAUCAUC
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ASP-siRNA (As strand): | AAGAUUUUCUGUGAUCAUC
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Mismatch position in siRNA: | U10A |
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Mutant allele (5'->3'): | UUCUAAAAGACACUAGUAG
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ASP-siRNA (As strand): | AAGAUUUUCUGUGAUCAUC
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ASP-siRNA efficacy for mutant allele: | 20
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Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
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ASP-siRNA (As strand): | AAGAUUUUCUGUGAUCAUC
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ASP-siRNA efficacy for wild allele: | 20
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Percentage discrimination for ASP-siRNA: | 0
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Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
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Wild siRNA (As strand): | AAGAUUUUCAGUGAUCAUC
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Wild sirna efficacy for wild allele: | 80
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Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
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ASP-siRNA (As strand): | AAGAUUUUCUGUGAUCAUC
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Wild sirna efficacy for mutant allele: | 20
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Percentage discrimination for wild siRNA: | 60
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| Concentration: | 20 nmol |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P37840 |
| Target sequence: | TCGAGTGGGGCATAGTCATTTCTAAAAGTCACTAGTAGAAAGTATAATTTCAAGATTACTAGT |
| Genbank Accesion: | NM_001146055.1 |
| Cell-line: | HeLa |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine2000 |
| siRNA expression method: | Sigma-Aldrich |
| Post-transfection duration: | 24 hours |
| OMID ID: | 168600 |
| KEGG disease ID: | H00057 |
| Reference: | 25965551 |
