| |
|---|
|
siRNA Id: | aspsirna3015
|
|
siRNA Name: | siSNCA_T2-8
|
| Gene: | Synuclein, alpha (SNCA) |
| Disease: | Parkinsons disease (PD) |
|
Wild allele/target (5'->3'): | NA
|
|
Mutant allele/target (5'->3'): | UUAUGUGGAUACAAAUUCU
|
|
Target Mutation: | SNCA locus triplication
|
|
Wild siRNA (As strand): | NA
|
|
ASP-siRNA (As strand): | AAUACACCUAUGUUUAAGA
|
|
Mismatch position in siRNA: | NA |
|
Mutant allele (5'->3'): | UUAUGUGGAUACAAAUUCU
|
|
ASP-siRNA (As strand): | AAUACACCUAUGUUUAAGA
|
|
ASP-siRNA efficacy for mutant allele: | 95
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand): | AAUACACCUAUGUUUAAGA
|
|
ASP-siRNA efficacy for wild allele: | 15
|
|
Percentage discrimination for ASP-siRNA: | 80
|
|
Wild allele (5'->3'): | NA
|
|
Wild siRNA (As strand): | NA
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand): | AAUACACCUAUGUUUAAGA
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 20 nmol |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P37840 |
| Target sequence: | TCGAGAGGATTTATGTGGATACAAATTCTCCTTTAAAGTGTTTCTTCCCTTAATATTTATCTGACTTACTAGT |
| Genbank Accesion: | NM_001146055.1 |
| Cell-line: | S2 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine2000 |
| siRNA expression method: | Sigma-Aldrich |
| Post-transfection duration: | 24 hours |
| OMID ID: | 168600 |
| KEGG disease ID: | H00057 |
| Reference: | 25965551 |
