aspsirna3043 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3043
siRNA Name: P9
Gene:Ataxin-3 (ATXN3)
Disease:Machado-Joseph Disease (MJD)/Spinocerebellar ataxia type 3 (SCA3)
Wild allele/target (5'->3'): CAGCAGCAGCAGCAGCAGC
Mutant allele/target (5'->3'):CAGCAGCAGCUGCAGCAGC
Target Mutation: c.892_894CAG(8_36) (p.Gln298_Gln305=)
Wild siRNA (As strand):GUCGUCGUCGUCGUCGUCG
ASP-siRNA (As strand):GUCGUCGUCGACGUCGUCG
Mismatch position in siRNA: A9U
Mutant allele (5'->3'): CAGCAGCAGCUGCAGCAGC
ASP-siRNA (As strand):GUCGUCGUCGACGUCGUCG
ASP-siRNA efficacy for mutant allele:78
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGC
ASP-siRNA (As strand):GUCGUCGUCGACGUCGUCG
ASP-siRNA efficacy for wild allele:20
Percentage discrimination for ASP-siRNA:58
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGC
Wild siRNA (As strand):GUCGUCGUCGUCGUCGUCG
Wild sirna efficacy for wild allele:70
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGC
ASP-siRNA (As strand):GUCGUCGUCGACGUCGUCG
Wild sirna efficacy for mutant allele:50
Percentage discrimination for wild siRNA:20
Concentration:12nM
Orientation:Antisense
Mismatch in:siRNA
UniProt ID: P54252
Target sequence: CAGCAGCAGCAGCAGCAGCAGCAGGGGGACCTATCAGGACAGAGTT
Genbank Accesion: NM_004993.5
Cell-line: Fibroblast
Experimental technique used: RT-PCR
Transfection method: RNAiMAX
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 24 hours
OMID ID: 109150
KEGG disease ID:H00063
Reference:21294677

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