| |
|---|
|
siRNA Id: | aspsirna3049
|
|
siRNA Name: | TGFBI-Arg124Cys-3
|
| Gene: | Transforming growth factor beta induced (TGFBI) |
| Disease: | Lattice corneal dystrophy type I (LCDI) |
|
Wild allele/target (5'->3'): | UCAGCUGUACACGGACCGC
|
|
Mutant allele/target (5'->3'): | UCAGCUGUACACGGACUGC
|
|
Target Mutation: | c.370C>T,p.Arg124Cys
|
|
Wild siRNA (As strand): | AGUCGACAUGUGCCUGGCG
|
|
ASP-siRNA (As strand): | AGUCGACAUGUGCCUGACG
|
|
Mismatch position in siRNA: | A3G |
|
Mutant allele (5'->3'): | UCAGCUGUACACGGACUGC
|
|
ASP-siRNA (As strand): | AGUCGACAUGUGCCUGACG
|
|
ASP-siRNA efficacy for mutant allele: | 45
|
|
Wild allele (5'->3'): | UCAGCUGUACACGGACCGC
|
|
ASP-siRNA (As strand): | AGUCGACAUGUGCCUGACG
|
|
ASP-siRNA efficacy for wild allele: | 40
|
|
Percentage discrimination for ASP-siRNA: | 5
|
|
Wild allele (5'->3'): | UCAGCUGUACACGGACCGC
|
|
Wild siRNA (As strand): | AGUCGACAUGUGCCUGGCG
|
|
Wild sirna efficacy for wild allele: | 50
|
|
Wild allele (5'->3'): | UCAGCUGUACACGGACCGC
|
|
ASP-siRNA (As strand): | AGUCGACAUGUGCCUGACG
|
|
Wild sirna efficacy for mutant allele: | 45
|
|
Percentage discrimination for wild siRNA: | 5
|
| Concentration: | 6.25 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q15582 |
| Target sequence: | ACUCAGCUGUACACGGACC |
| Genbank Accesion: | NM_000358.2 |
| Cell-line: | AD293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | MWG Biotech |
| Post-transfection duration: | 24 hours |
| OMID ID: | 122200 |
| KEGG disease ID: | H00956 |
| Reference: | 24425855 |
