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siRNA Id: | aspsirna3054
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siRNA Name: | TGFBI-Arg124Cys-8
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Gene: | Transforming growth factor beta induced (TGFBI) |
Disease: | Lattice corneal dystrophy type I (LCDI) |
Wild allele/target (5'->3'): | UGUACACGGACCGCACGGA
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Mutant allele/target (5'->3'): | UGUACACGGACUGCACGGA
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Target Mutation: | c.370C>T,p.Arg124Cys
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Wild siRNA (As strand): | ACAUGUGCCUGGCGUGCCU
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ASP-siRNA (As strand): | ACAUGUGCCUGACGUGCCU
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Mismatch position in siRNA: | A8G |
Mutant allele (5'->3'): | UGUACACGGACUGCACGGA
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ASP-siRNA (As strand): | ACAUGUGCCUGACGUGCCU
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ASP-siRNA efficacy for mutant allele: | 60
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Wild allele (5'->3'): | UGUACACGGACCGCACGGA
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ASP-siRNA (As strand): | ACAUGUGCCUGACGUGCCU
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ASP-siRNA efficacy for wild allele: | 44
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Percentage discrimination for ASP-siRNA: | 16
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Wild allele (5'->3'): | UGUACACGGACCGCACGGA
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Wild siRNA (As strand): | ACAUGUGCCUGGCGUGCCU
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Wild sirna efficacy for wild allele: | 50
|
Wild allele (5'->3'): | UGUACACGGACCGCACGGA
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ASP-siRNA (As strand): | ACAUGUGCCUGACGUGCCU
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Wild sirna efficacy for mutant allele: | 45
|
Percentage discrimination for wild siRNA: | 5
|
Concentration: | 6.25 nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | Q15582 |
Target sequence: | ACUCAGCUGUACACGGACC |
Genbank Accesion: | NM_000358.2 |
Cell-line: | AD293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | MWG Biotech |
Post-transfection duration: | 24 hours |
OMID ID: | 122200 |
KEGG disease ID: | H00956 |
Reference: | 24425855 |