aspsirna3054 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3054
siRNA Name: TGFBI-Arg124Cys-8
Gene:Transforming growth factor beta induced (TGFBI)
Disease:Lattice corneal dystrophy type I (LCDI)
Wild allele/target (5'->3'): UGUACACGGACCGCACGGA
Mutant allele/target (5'->3'):UGUACACGGACUGCACGGA
Target Mutation: c.370C>T,p.Arg124Cys
Wild siRNA (As strand):ACAUGUGCCUGGCGUGCCU
ASP-siRNA (As strand):ACAUGUGCCUGACGUGCCU
Mismatch position in siRNA: A8G
Mutant allele (5'->3'): UGUACACGGACUGCACGGA
ASP-siRNA (As strand):ACAUGUGCCUGACGUGCCU
ASP-siRNA efficacy for mutant allele:60
Wild allele (5'->3'): UGUACACGGACCGCACGGA
ASP-siRNA (As strand):ACAUGUGCCUGACGUGCCU
ASP-siRNA efficacy for wild allele:44
Percentage discrimination for ASP-siRNA:16
Wild allele (5'->3'): UGUACACGGACCGCACGGA
Wild siRNA (As strand):ACAUGUGCCUGGCGUGCCU
Wild sirna efficacy for wild allele:50
Wild allele (5'->3'): UGUACACGGACCGCACGGA
ASP-siRNA (As strand):ACAUGUGCCUGACGUGCCU
Wild sirna efficacy for mutant allele:45
Percentage discrimination for wild siRNA:5
Concentration:6.25 nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q15582
Target sequence: ACUCAGCUGUACACGGACC
Genbank Accesion: NM_000358.2
Cell-line: AD293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: MWG Biotech
Post-transfection duration: 24 hours
OMID ID: 122200
KEGG disease ID:H00956
Reference:24425855

.
.
.
.
.
.
.
.
.
.
.

.

.

.