aspsirna3057 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3057
siRNA Name: TGFBI-Arg124Cys-11
Gene:Transforming growth factor beta induced (TGFBI)
Disease:Lattice corneal dystrophy type I (LCDI)
Wild allele/target (5'->3'): ACACGGACCGCACGGAGAA
Mutant allele/target (5'->3'):ACACGGACUGCACGGAGAA
Target Mutation: c.370C>T,p.Arg124Cys
Wild siRNA (As strand):UGUGCCUGGCGUGCCUCUU
ASP-siRNA (As strand):UGUGCCUGACGUGCCUCUU
Mismatch position in siRNA: A11G
Mutant allele (5'->3'): ACACGGACUGCACGGAGAA
ASP-siRNA (As strand):UGUGCCUGACGUGCCUCUU
ASP-siRNA efficacy for mutant allele:65
Wild allele (5'->3'): ACACGGACCGCACGGAGAA
ASP-siRNA (As strand):UGUGCCUGACGUGCCUCUU
ASP-siRNA efficacy for wild allele:35
Percentage discrimination for ASP-siRNA:30
Wild allele (5'->3'): ACACGGACCGCACGGAGAA
Wild siRNA (As strand):UGUGCCUGGCGUGCCUCUU
Wild sirna efficacy for wild allele:50
Wild allele (5'->3'): ACACGGACCGCACGGAGAA
ASP-siRNA (As strand):UGUGCCUGACGUGCCUCUU
Wild sirna efficacy for mutant allele:45
Percentage discrimination for wild siRNA:5
Concentration:6.25 nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q15582
Target sequence: ACUCAGCUGUACACGGACC
Genbank Accesion: NM_000358.2
Cell-line: AD293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: MWG Biotech
Post-transfection duration: 24 hours
OMID ID: 122200
KEGG disease ID:H00956
Reference:24425855

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