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siRNA Id: | aspsirna3064
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siRNA Name: | TGFBI-Arg124Cys-18
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| Gene: | Transforming growth factor beta induced (TGFBI) |
| Disease: | Lattice corneal dystrophy type I (LCDI) |
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Wild allele/target (5'->3'): | CCGCACGGAGAAGCUGAGG
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Mutant allele/target (5'->3'): | CUGCACGGAGAAGCUGAGG
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Target Mutation: | c.370C>T,p.Arg124Cys
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Wild siRNA (As strand): | GGCGUGCCUCUUCGACUCC
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ASP-siRNA (As strand): | GACGUGCCUCUUCGACUCC
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Mismatch position in siRNA: | A18G |
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Mutant allele (5'->3'): | CUGCACGGAGAAGCUGAGG
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ASP-siRNA (As strand): | GACGUGCCUCUUCGACUCC
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ASP-siRNA efficacy for mutant allele: | 50
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Wild allele (5'->3'): | CCGCACGGAGAAGCUGAGG
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ASP-siRNA (As strand): | GACGUGCCUCUUCGACUCC
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 20
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Wild allele (5'->3'): | CCGCACGGAGAAGCUGAGG
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Wild siRNA (As strand): | GGCGUGCCUCUUCGACUCC
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Wild sirna efficacy for wild allele: | 50
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Wild allele (5'->3'): | CCGCACGGAGAAGCUGAGG
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ASP-siRNA (As strand): | GACGUGCCUCUUCGACUCC
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Wild sirna efficacy for mutant allele: | 45
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Percentage discrimination for wild siRNA: | 5
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| Concentration: | 6.25 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q15582 |
| Target sequence: | ACUCAGCUGUACACGGACC |
| Genbank Accesion: | NM_000358.2 |
| Cell-line: | AD293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | MWG Biotech |
| Post-transfection duration: | 24 hours |
| OMID ID: | 122200 |
| KEGG disease ID: | H00956 |
| Reference: | 24425855 |
