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siRNA Id: | aspsirna3076
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siRNA Name: | mt1-siRNA
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| Gene: | Tumor protein p53 |
| Disease: | Cancer |
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Wild allele/target (5'->3'): | GCAUGAACCGGAGGCCCAU
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Mutant allele/target (5'->3'): | GCAUGAACUGGAGGCCCAU
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Target Mutation: | c.944C>T,p.R249W
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Wild siRNA (As strand): | CGUACUUGGCCUCCGGGUA
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ASP-siRNA (As strand): | CGUACUUGACCUCCGGGUA
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Mismatch position in siRNA: | A11G |
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Mutant allele (5'->3'): | GCAUGAACUGGAGGCCCAU
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ASP-siRNA (As strand): | CGUACUUGACCUCCGGGUA
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ASP-siRNA efficacy for mutant allele: | 80
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Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
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ASP-siRNA (As strand): | CGUACUUGACCUCCGGGUA
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 50
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Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
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Wild siRNA (As strand): | CGUACUUGGCCUCCGGGUA
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Wild sirna efficacy for wild allele: | 70
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Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
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ASP-siRNA (As strand): | CGUACUUGACCUCCGGGUA
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Wild sirna efficacy for mutant allele: | 60
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Percentage discrimination for wild siRNA: | 10
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| Concentration: | 2 microg |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P04637 |
| Target sequence: | GCATGAACCGGAGGCCCATCCTCACCATCATCA |
| Genbank Accesion: | NM_000546 |
| Cell-line: | U2OS |
| Experimental technique used: | Luciferase reporter assay |
| Transfection method: | Lipofectamine |
| siRNA expression method: | MWG Biotech |
| Post-transfection duration: | 24 hours |
| OMID ID: | 191170 |
| KEGG disease ID: | H00881 |
| Reference: | 12403821 |
