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siRNA Id: | aspsirna3086
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siRNA Name: | R273H-#3
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| Gene: | Tumor protein p53 |
| Disease: | Cancer |
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Wild allele/target (5'->3'): | GAGGUGCGUGUUUGUGCCU
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Mutant allele/target (5'->3'): | GAGGUGCAUGUUUGUGCCU
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Target Mutation: | NC_000005.10:g.144594367C>A
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Wild siRNA (As strand): | CUCCACGCACAAACACGGA
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ASP-siRNA (As strand): | CUCCACGUACAAACACGGA
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Mismatch position in siRNA: | U12C |
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Mutant allele (5'->3'): | GAGGUGCAUGUUUGUGCCU
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ASP-siRNA (As strand): | CUCCACGUACAAACACGGA
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ASP-siRNA efficacy for mutant allele: | 78
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Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
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ASP-siRNA (As strand): | CUCCACGUACAAACACGGA
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ASP-siRNA efficacy for wild allele: | 20
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Percentage discrimination for ASP-siRNA: | 58
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Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
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Wild siRNA (As strand): | CUCCACGCACAAACACGGA
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Wild sirna efficacy for wild allele: | 70
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Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
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ASP-siRNA (As strand): | CUCCACGUACAAACACGGA
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Wild sirna efficacy for mutant allele: | 50
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Percentage discrimination for wild siRNA: | 20
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| Concentration: | 80 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P04637 |
| Target sequence: | CUUUGAGGUGCGTGUUUGUGCCUGUCCU |
| Genbank Accesion: | NM_000546.5 |
| Cell-line: | SW620 |
| Experimental technique used: | Western blot |
| Transfection method: | INTERFERin |
| siRNA expression method: | Integrated DNA Technology |
| Post-transfection duration: | 24 hours |
| OMID ID: | 191170 |
| KEGG disease ID: | H00881 |
| Reference: | 26700961 |
