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siRNA Id: | aspsirna3105
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siRNA Name: | R175H-#3
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Gene: | Tumor protein p53 |
Disease: | Cancer |
Wild allele/target (5'->3'): | AGGCGCUGCCCCCACCAUG
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Mutant allele/target (5'->3'): | AGGCACUGCCCCCACCAUG
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Target Mutation: | c.524G>A ,p.Arg175His
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Wild siRNA (As strand): | UCCGCGACGGGGGUGGUAC
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ASP-siRNA (As strand): | UCCGUGACGGGGGUGGUAC
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Mismatch position in siRNA: | U15C |
Mutant allele (5'->3'): | AGGCACUGCCCCCACCAUG
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ASP-siRNA (As strand): | UCCGUGACGGGGGUGGUAC
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ASP-siRNA efficacy for mutant allele: | 60
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Wild allele (5'->3'): | AGGCGCUGCCCCCACCAUG
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ASP-siRNA (As strand): | UCCGUGACGGGGGUGGUAC
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ASP-siRNA efficacy for wild allele: | 45
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Percentage discrimination for ASP-siRNA: | 15
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Wild allele (5'->3'): | AGGCGCUGCCCCCACCAUG
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Wild siRNA (As strand): | UCCGCGACGGGGGUGGUAC
|
Wild sirna efficacy for wild allele: | 60
|
Wild allele (5'->3'): | AGGCGCUGCCCCCACCAUG
|
ASP-siRNA (As strand): | UCCGUGACGGGGGUGGUAC
|
Wild sirna efficacy for mutant allele: | 45
|
Percentage discrimination for wild siRNA: | 15
|
Concentration: | 80 nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P04637 |
Target sequence: | UGACGGAGGUUGUGAGGCGCUGCCCCCACCAUGA |
Genbank Accesion: | NM_000546.5 |
Cell-line: | CAL33 |
Experimental technique used: | Western blot |
Transfection method: | INTERFERin |
siRNA expression method: | Integrated DNA Technology |
Post-transfection duration: | 24 hours |
OMID ID: | 191170 |
KEGG disease ID: | H00881 |
Reference: | 26700961 |