| |
siRNA Id: | aspsirna3132
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siRNA Name: | YWT-Ds
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Gene: | Type 1 ryanodine receptor (RyR1) |
Disease: | Malignanat Hyperthermia (MH) |
Wild allele/target (5'->3'): | NA
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Mutant allele/target (5'->3'): | UUGUGAACCUGCUCUAUGA
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Target Mutation: | c.1565A>C,p.Tyr522Ser
|
Wild siRNA (As strand): | NA
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ASP-siRNA (As strand): | AACACUUGGACGAGAUACU
|
Mismatch position in siRNA: | NA |
Mutant allele (5'->3'): | UUGUGAACCUGCUCUAUGA
|
ASP-siRNA (As strand): | AACACUUGGACGAGAUACU
|
ASP-siRNA efficacy for mutant allele: | 80
|
Wild allele (5'->3'): | NA
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ASP-siRNA (As strand): | AACACUUGGACGAGAUACU
|
ASP-siRNA efficacy for wild allele: | 35
|
Percentage discrimination for ASP-siRNA: | 45
|
Wild allele (5'->3'): | NA
|
Wild siRNA (As strand): | NA
|
Wild sirna efficacy for wild allele: | 40
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand): | AACACUUGGACGAGAUACU
|
Wild sirna efficacy for mutant allele: | 19
|
Percentage discrimination for wild siRNA: | 21
|
Concentration: | 200pmol |
Orientation: | Sense |
Mismatch in: | NA |
UniProt ID: | P21817 |
Target sequence: | CCTGCTCAGCGAACTTCTA |
Genbank Accesion: | NM_000540.2 |
Cell-line: | HEK293 |
Experimental technique used: | Western blot |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 48 hours |
OMID ID: | 117000 |
KEGG disease ID: | H00699 |
Reference: | 23152933 |