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siRNA Id: | aspsirna3133
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siRNA Name: | YS-Dc
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| Gene: | Type 1 ryanodine receptor (RyR1) |
| Disease: | Malignanat Hyperthermia (MH) |
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Wild allele/target (5'->3'): | CCUGCUCUAUGAACUUCUA
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Mutant allele/target (5'->3'): | CCUGCUCAGCGAACUUCUA
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Target Mutation: | c.1565A>C,p.Tyr522Ser
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Wild siRNA (As strand): | GGACGAGAUACUUGAAGAU
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ASP-siRNA (As strand): | GGACGAGUCGCUUGAAGAU
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Mismatch position in siRNA: | U12A,C11U,G10A |
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Mutant allele (5'->3'): | CCUGCUCAGCGAACUUCUA
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ASP-siRNA (As strand): | GGACGAGUCGCUUGAAGAU
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ASP-siRNA efficacy for mutant allele: | 45
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Wild allele (5'->3'): | CCUGCUCUAUGAACUUCUA
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ASP-siRNA (As strand): | GGACGAGUCGCUUGAAGAU
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ASP-siRNA efficacy for wild allele: | 20
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Percentage discrimination for ASP-siRNA: | 25
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Wild allele (5'->3'): | CCUGCUCUAUGAACUUCUA
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Wild siRNA (As strand): | GGACGAGAUACUUGAAGAU
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Wild sirna efficacy for wild allele: | 40
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Wild allele (5'->3'): | CCUGCUCUAUGAACUUCUA
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ASP-siRNA (As strand): | GGACGAGUCGCUUGAAGAU
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Wild sirna efficacy for mutant allele: | 19
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Percentage discrimination for wild siRNA: | 21
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| Concentration: | 200pmol |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P21817 |
| Target sequence: | TTGTGAACCTGCTCAGCGA |
| Genbank Accesion: | NM_000540.2 |
| Cell-line: | HEK293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| OMID ID: | 117000 |
| KEGG disease ID: | H00699 |
| Reference: | 23152933 |
