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siRNA Id: | aspsirna3135
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siRNA Name: | siRNA-T2
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| Gene: | tumor protein p63 (TP63) |
| Disease: | Ectrodactily-ectodermal dysplasia and cleft lip/palate (EEC) |
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Wild allele/target (5'->3'): | CCGGAUCUGUGCUUGCCCA
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Mutant allele/target (5'->3'): | CUGGAUCUGUGCUUGCCCA
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Target Mutation: | c.727C>T, p.Arg243Trp
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Wild siRNA (As strand): | GGCCUAGACACGAACGGGU
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ASP-siRNA (As strand): | GACCUAGACACGAACGGGU
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Mismatch position in siRNA: | A18G |
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Mutant allele (5'->3'): | CUGGAUCUGUGCUUGCCCA
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ASP-siRNA (As strand): | GACCUAGACACGAACGGGU
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ASP-siRNA efficacy for mutant allele: | 85
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Wild allele (5'->3'): | CCGGAUCUGUGCUUGCCCA
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ASP-siRNA (As strand): | GACCUAGACACGAACGGGU
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ASP-siRNA efficacy for wild allele: | 93
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Percentage discrimination for ASP-siRNA: | -8
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Wild allele (5'->3'): | CCGGAUCUGUGCUUGCCCA
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Wild siRNA (As strand): | GGCCUAGACACGAACGGGU
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Wild sirna efficacy for wild allele: | 60
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Wild allele (5'->3'): | CCGGAUCUGUGCUUGCCCA
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ASP-siRNA (As strand): | GACCUAGACACGAACGGGU
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Wild sirna efficacy for mutant allele: | 70
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Percentage discrimination for wild siRNA: | -10
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| Concentration: | 40 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q9H3D4 |
| Target sequence: | TCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCC |
| Genbank Accesion: | NM_001114980 |
| Cell-line: | HEK-293E |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| OMID ID: | 604292 |
| KEGG disease ID: | NA |
| Reference: | 27195674 |
