| |
|---|
|
siRNA Id: | aspsirna3165
|
|
siRNA Name: | mi268.5 (3->5')
|
| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
|
Wild allele/target (5'->3'): | UCCCUCCUCGAGGCUGGGUGUU
|
|
Mutant allele/target (5'->3'): | UCCCUCCUGCAGGCUGGCUGUU
|
|
Target Mutation: | rs362268
|
|
Wild siRNA (As strand): | AGGGAGGACGUCCGACCCACAA
|
|
ASP-siRNA (As strand): | AGGGAGGACGUCCGACCGACAA
|
|
Mismatch position in siRNA: | G5C |
|
Mutant allele (5'->3'): | UCCCUCCUGCAGGCUGGCUGUU
|
|
ASP-siRNA (As strand): | AGGGAGGACGUCCGACCGACAA
|
|
ASP-siRNA efficacy for mutant allele: | 70
|
|
Wild allele (5'->3'): | UCCCUCCUCGAGGCUGGGUGUU
|
|
ASP-siRNA (As strand): | AGGGAGGACGUCCGACCGACAA
|
|
ASP-siRNA efficacy for wild allele: | 20
|
|
Percentage discrimination for ASP-siRNA: | 50
|
|
Wild allele (5'->3'): | UCCCUCCUCGAGGCUGGGUGUU
|
|
Wild siRNA (As strand): | AGGGAGGACGUCCGACCCACAA
|
|
Wild sirna efficacy for wild allele: | 45
|
|
Wild allele (5'->3'): | UCCCUCCUCGAGGCUGGGUGUU
|
|
ASP-siRNA (As strand): | AGGGAGGACGUCCGACCGACAA
|
|
Wild sirna efficacy for mutant allele: | 57
|
|
Percentage discrimination for wild siRNA: | -12
|
| Concentration: | 40 nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P42858 |
| Target sequence: | NA |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | HEK-293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 25849618 |
