aspsirna3170 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3170
siRNA Name: miCAG3 (5'->3')
Gene:Hungtintin (HTT)
Disease:Huntington disease (HD)
Wild allele/target (5'->3'): CAACGACGACGACGACGACGAC
Mutant allele/target (5'->3'):CAACGACGUUUACGACGACGAC
Target Mutation: (CAG)81
Wild siRNA (As strand):GUUGCUGCUGCUGCUGCUGCUG
ASP-siRNA (As strand):GUUGCUGCAAAUGCUGCUGCUG
Mismatch position in siRNA: A9U,A10G,A11C
Mutant allele (5'->3'): CAACGACGUUUACGACGACGAC
ASP-siRNA (As strand):GUUGCUGCAAAUGCUGCUGCUG
ASP-siRNA efficacy for mutant allele:45
Wild allele (5'->3'): CAACGACGACGACGACGACGAC
ASP-siRNA (As strand):GUUGCUGCAAAUGCUGCUGCUG
ASP-siRNA efficacy for wild allele:27
Percentage discrimination for ASP-siRNA:18
Wild allele (5'->3'): CAACGACGACGACGACGACGAC
Wild siRNA (As strand):GUUGCUGCUGCUGCUGCUGCUG
Wild sirna efficacy for wild allele:70
Wild allele (5'->3'): CAACGACGACGACGACGACGAC
ASP-siRNA (As strand):GUUGCUGCAAAUGCUGCUGCUG
Wild sirna efficacy for mutant allele:45
Percentage discrimination for wild siRNA:25
Concentration:40 nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P42858
Target sequence: NA
Genbank Accesion: NM_002111.7
Cell-line: HEK-293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
OMID ID: 143100
KEGG disease ID:H00059
Reference:25849618

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