aspsirna3171 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3171
siRNA Name: miCAG9
Gene:Hungtintin (HTT)
Disease:Huntington disease (HD)
Wild allele/target (5'->3'): CAACGACGACGACGACGACGAC
Mutant allele/target (5'->3'):CAACGACGUCGACGACGACGAC
Target Mutation: (CAG)81
Wild siRNA (As strand):GUUGCUGCUGCUGCUGCUGCUG
ASP-siRNA (As strand):GUUGCUGCAGCUGCUGCUGCUG
Mismatch position in siRNA: A9U
Mutant allele (5'->3'): CAACGACGUCGACGACGACGAC
ASP-siRNA (As strand):GUUGCUGCAGCUGCUGCUGCUG
ASP-siRNA efficacy for mutant allele:77
Wild allele (5'->3'): CAACGACGACGACGACGACGAC
ASP-siRNA (As strand):GUUGCUGCAGCUGCUGCUGCUG
ASP-siRNA efficacy for wild allele:50
Percentage discrimination for ASP-siRNA:27
Wild allele (5'->3'): CAACGACGACGACGACGACGAC
Wild siRNA (As strand):GUUGCUGCUGCUGCUGCUGCUG
Wild sirna efficacy for wild allele:45
Wild allele (5'->3'): CAACGACGACGACGACGACGAC
ASP-siRNA (As strand):GUUGCUGCAGCUGCUGCUGCUG
Wild sirna efficacy for mutant allele:57
Percentage discrimination for wild siRNA:-12
Concentration:40 nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P42858
Target sequence: NA
Genbank Accesion: NM_002111.7
Cell-line: HEK-293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
OMID ID: 143100
KEGG disease ID:H00059
Reference:25849618

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