aspsirna3178 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3178
siRNA Name: mi306.12
Gene:Hungtintin (HTT)
Disease:Huntington disease (HD)
Wild allele/target (5'->3'): UUGCCAGGUUACAGCUGCUCUU
Mutant allele/target (5'->3'):UUGCCAGGUUGCAGCUGCUCUU
Target Mutation: rs362306
Wild siRNA (As strand):AACGGUCCAAUGUCGACGAGAA
ASP-siRNA (As strand):AACGGUCCAACGUCGACGAGAA
Mismatch position in siRNA: C12U
Mutant allele (5'->3'): UUGCCAGGUUGCAGCUGCUCUU
ASP-siRNA (As strand):AACGGUCCAACGUCGACGAGAA
ASP-siRNA efficacy for mutant allele:90
Wild allele (5'->3'): UUGCCAGGUUACAGCUGCUCUU
ASP-siRNA (As strand):AACGGUCCAACGUCGACGAGAA
ASP-siRNA efficacy for wild allele:26
Percentage discrimination for ASP-siRNA:64
Wild allele (5'->3'): UUGCCAGGUUACAGCUGCUCUU
Wild siRNA (As strand):AACGGUCCAAUGUCGACGAGAA
Wild sirna efficacy for wild allele:45
Wild allele (5'->3'): UUGCCAGGUUACAGCUGCUCUU
ASP-siRNA (As strand):AACGGUCCAACGUCGACGAGAA
Wild sirna efficacy for mutant allele:57
Percentage discrimination for wild siRNA:-12
Concentration:40 nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P42858
Target sequence: NA
Genbank Accesion: NM_002111.7
Cell-line: Transgenic mouse model
Experimental technique used: qRT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 3 weeks
OMID ID: 143100
KEGG disease ID:H00059
Reference:25849618

.
.
.
.
.
.
.
.
.
.
.

.

.

.