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siRNA Id: | aspsirna3179
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siRNA Name: | siSN
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| Gene: | Cyclin-dependent kinase 2 (CDK2) |
| Disease: | NA |
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Wild allele/target (5'->3'): | GAAUUGAAUCACCCGAACA
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Mutant allele/target (5'->3'): | GAAUUGAAUCACCCGAACA
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Target Mutation: | NA
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Wild siRNA (As strand): | CUUAACUUAGUGGGCUUGU
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ASP-siRNA (As strand): | CUUAACUUAGUGGGCUUGU
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Mismatch position in siRNA: | NA |
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Mutant allele (5'->3'): | GAAUUGAAUCACCCGAACA
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ASP-siRNA (As strand): | CUUAACUUAGUGGGCUUGU
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ASP-siRNA efficacy for mutant allele: | 75
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Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
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ASP-siRNA (As strand): | CUUAACUUAGUGGGCUUGU
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ASP-siRNA efficacy for wild allele: | 0
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Percentage discrimination for ASP-siRNA: | 75
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Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
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Wild siRNA (As strand): | CUUAACUUAGUGGGCUUGU
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Wild sirna efficacy for wild allele: | 80
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Wild allele (5'->3'): | GAAUUGAAUCACCCGAACA
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ASP-siRNA (As strand): | CUUAACUUAGUGGGCUUGU
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Wild sirna efficacy for mutant allele: | 100
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Percentage discrimination for wild siRNA: | -20
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| Concentration: | 25 nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P24941 |
| Target sequence: | NA |
| Genbank Accesion: | NA |
| Cell-line: | hTERT-RPE1 |
| Experimental technique used: | qRT-PCR |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Life Technologies |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 25586224 |
