| |
siRNA Id: | aspsirna3190
|
siRNA Name: | siRNA-j
|
Gene: | tumor protein p63 (TP63) |
Disease: | Ectrodactyly-Ectodermal Dysplasia-Clefting syndrome 3 (EEC3) |
Wild allele/target (5'->3'): | AGUUUCGACGUGUCCUUCC
|
Mutant allele/target (5'->3'): | AGUUUCGACAUGUCCUUCC
|
Target Mutation: | p.Arg132His,c.394G>A
|
Wild siRNA (As strand): | UCAAAGCUGCACAGGAAGG
|
ASP-siRNA (As strand): | UCAAAGCUGUACAGGAAGG
|
Mismatch position in siRNA: | U10C |
Mutant allele (5'->3'): | AGUUUCGACAUGUCCUUCC
|
ASP-siRNA (As strand): | UCAAAGCUGUACAGGAAGG
|
ASP-siRNA efficacy for mutant allele: | 60
|
Wild allele (5'->3'): | AGUUUCGACGUGUCCUUCC
|
ASP-siRNA (As strand): | UCAAAGCUGUACAGGAAGG
|
ASP-siRNA efficacy for wild allele: | 38
|
Percentage discrimination for ASP-siRNA: | 22
|
Wild allele (5'->3'): | AGUUUCGACGUGUCCUUCC
|
Wild siRNA (As strand): | UCAAAGCUGCACAGGAAGG
|
Wild sirna efficacy for wild allele: | 45
|
Wild allele (5'->3'): | AGUUUCGACGUGUCCUUCC
|
ASP-siRNA (As strand): | UCAAAGCUGUACAGGAAGG
|
Wild sirna efficacy for mutant allele: | 65
|
Percentage discrimination for wild siRNA: | -20
|
Concentration: | 100 microM |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | Q9H3D4 |
Target sequence: | CATCCCCTCCAACACCGACTACCCAGGCCCGCACAGTTTCGACG |
Genbank Accesion: | NM_001114980.1 |
Cell-line: | HEK293T |
Experimental technique used: | Western blot |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Exiqon |
Post-transfection duration: | 72 hours |
OMID ID: | 604292 |
KEGG disease ID: | NA |
Reference: | 26891374 |