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siRNA Id: | aspsirna3197
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siRNA Name: | siRNA-q
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| Gene: | tumor protein p63 (TP63) |
| Disease: | Ectrodactyly-Ectodermal Dysplasia-Clefting syndrome 3 (EEC3) |
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Wild allele/target (5'->3'): | ACGUGUCCUUCCAGCAGUC
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Mutant allele/target (5'->3'): | ACAUGUCCUUCCAGCAGUC
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Target Mutation: | p.Arg132His,c.394G>A
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Wild siRNA (As strand): | UGCACAGGAAGGUCGUCAG
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ASP-siRNA (As strand): | UGUACAGGAAGGUCGUCAG
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Mismatch position in siRNA: | U17C |
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Mutant allele (5'->3'): | ACAUGUCCUUCCAGCAGUC
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ASP-siRNA (As strand): | UGUACAGGAAGGUCGUCAG
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ASP-siRNA efficacy for mutant allele: | 40
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Wild allele (5'->3'): | ACGUGUCCUUCCAGCAGUC
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ASP-siRNA (As strand): | UGUACAGGAAGGUCGUCAG
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 10
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Wild allele (5'->3'): | ACGUGUCCUUCCAGCAGUC
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Wild siRNA (As strand): | UGCACAGGAAGGUCGUCAG
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Wild sirna efficacy for wild allele: | 45
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Wild allele (5'->3'): | ACGUGUCCUUCCAGCAGUC
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ASP-siRNA (As strand): | UGUACAGGAAGGUCGUCAG
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Wild sirna efficacy for mutant allele: | 65
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Percentage discrimination for wild siRNA: | -20
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| Concentration: | 100 microM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q9H3D4 |
| Target sequence: | CATCCCCTCCAACACCGACTACCCAGGCCCGCACAGTTTCGACG |
| Genbank Accesion: | NM_001114980.1 |
| Cell-line: | HEK293T |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Exiqon |
| Post-transfection duration: | 72 hours |
| OMID ID: | 604292 |
| KEGG disease ID: | NA |
| Reference: | 26891374 |
