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siRNA Id: | aspsirna3201
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siRNA Name: | siN159D-2
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| Gene: | Keratin 75 (KRT75) |
| Disease: | Hair shaft blebbing |
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Wild allele/target (5'->3'): | CAGAUCAAGACUCUGAACAACA
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Mutant allele/target (5'->3'): | CAGAUCAAGACUCUGAAC---A
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Target Mutation: | c.545_547del,p.N159del
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Wild siRNA (As strand): | GUCUAGUUCUGAGACUUGUUGU
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ASP-siRNA (As strand): | GUCUAGUUCUGAGACUUG---U
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Mismatch position in siRNA: | del_19A,20A,21C, |
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Mutant allele (5'->3'): | CAGAUCAAGACUCUGAAC---A
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ASP-siRNA (As strand): | GUCUAGUUCUGAGACUUG---U
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ASP-siRNA efficacy for mutant allele: | 65
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Wild allele (5'->3'): | CAGAUCAAGACUCUGAACAACA
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ASP-siRNA (As strand): | GUCUAGUUCUGAGACUUG---U
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ASP-siRNA efficacy for wild allele: | 24
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Percentage discrimination for ASP-siRNA: | 41
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Wild allele (5'->3'): | CAGAUCAAGACUCUGAACAACA
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Wild siRNA (As strand): | GUCUAGUUCUGAGACUUGUUGU
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Wild sirna efficacy for wild allele: | 50
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Wild allele (5'->3'): | CAGAUCAAGACUCUGAACAACA
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ASP-siRNA (As strand): | GUCUAGUUCUGAGACUUG---U
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Wild sirna efficacy for mutant allele: | 42
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Percentage discrimination for wild siRNA: | 8
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| Concentration: | 15 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | O95678 |
| Target sequence: | ACGGGAGCAGATCAAGACTCTGAACAACAAGTTCGCCTCCTTC |
| Genbank Accesion: | NM_004693.2 |
| Cell-line: | HEK293T |
| Experimental technique used: | qRT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Thermo Fisher |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 26763422 |
