| |
|---|
|
siRNA Id: | aspsirna3209
|
|
siRNA Name: | siN159D-10
|
| Gene: | Keratin 75 (KRT75) |
| Disease: | Hair shaft blebbing |
|
Wild allele/target (5'->3'): | GACUCUGAACAACAAGUUCGCC
|
|
Mutant allele/target (5'->3'): | GACUCUGAAC---AAGUUCGCC
|
|
Target Mutation: | c.545_547del,p.N159del
|
|
Wild siRNA (As strand): | CUGAGACUUGUUGUUCAAGCGG
|
|
ASP-siRNA (As strand): | CUGAGACUUG---UUCAAGCGG
|
|
Mismatch position in siRNA: | del_11A,12A,13C, |
|
Mutant allele (5'->3'): | GACUCUGAAC---AAGUUCGCC
|
|
ASP-siRNA (As strand): | CUGAGACUUG---UUCAAGCGG
|
|
ASP-siRNA efficacy for mutant allele: | 35
|
|
Wild allele (5'->3'): | GACUCUGAACAACAAGUUCGCC
|
|
ASP-siRNA (As strand): | CUGAGACUUG---UUCAAGCGG
|
|
ASP-siRNA efficacy for wild allele: | 58
|
|
Percentage discrimination for ASP-siRNA: | -23
|
|
Wild allele (5'->3'): | GACUCUGAACAACAAGUUCGCC
|
|
Wild siRNA (As strand): | CUGAGACUUGUUGUUCAAGCGG
|
|
Wild sirna efficacy for wild allele: | 50
|
|
Wild allele (5'->3'): | GACUCUGAACAACAAGUUCGCC
|
|
ASP-siRNA (As strand): | CUGAGACUUG---UUCAAGCGG
|
|
Wild sirna efficacy for mutant allele: | 42
|
|
Percentage discrimination for wild siRNA: | 8
|
| Concentration: | 15 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | O95678 |
| Target sequence: | ACGGGAGCAGATCAAGACTCTGAACAACAAGTTCGCCTCCTTC |
| Genbank Accesion: | NM_004693.2 |
| Cell-line: | HEK293T |
| Experimental technique used: | qRT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Thermo Fisher |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 26763422 |
