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siRNA Id: | aspsirna3214
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siRNA Name: | siN159D-15
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| Gene: | Keratin 75 (KRT75) |
| Disease: | Hair shaft blebbing |
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Wild allele/target (5'->3'): | UGAACAACAAGUUCGCCUCCUU
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Mutant allele/target (5'->3'): | UGAAC---AAGUUCGCCUCCUU
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Target Mutation: | c.545_547del,p.N159del
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Wild siRNA (As strand): | ACUUGUUGUUCAAGCGGAGGAA
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ASP-siRNA (As strand): | ACUUG---UUCAAGCGGAGGAA
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Mismatch position in siRNA: | del_6A,7A,8C, |
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Mutant allele (5'->3'): | UGAAC---AAGUUCGCCUCCUU
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ASP-siRNA (As strand): | ACUUG---UUCAAGCGGAGGAA
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ASP-siRNA efficacy for mutant allele: | 34
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Wild allele (5'->3'): | UGAACAACAAGUUCGCCUCCUU
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ASP-siRNA (As strand): | ACUUG---UUCAAGCGGAGGAA
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ASP-siRNA efficacy for wild allele: | 40
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Percentage discrimination for ASP-siRNA: | -6
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Wild allele (5'->3'): | UGAACAACAAGUUCGCCUCCUU
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Wild siRNA (As strand): | ACUUGUUGUUCAAGCGGAGGAA
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Wild sirna efficacy for wild allele: | 50
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Wild allele (5'->3'): | UGAACAACAAGUUCGCCUCCUU
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ASP-siRNA (As strand): | ACUUG---UUCAAGCGGAGGAA
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Wild sirna efficacy for mutant allele: | 42
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Percentage discrimination for wild siRNA: | 8
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| Concentration: | 15 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | O95678 |
| Target sequence: | ACGGGAGCAGATCAAGACTCTGAACAACAAGTTCGCCTCCTTC |
| Genbank Accesion: | NM_004693.2 |
| Cell-line: | HEK293T |
| Experimental technique used: | qRT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Thermo Fisher |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 26763422 |
