aspsirna3221 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3221
siRNA Name: siN159D-3
Gene:Keratin 75 (KRT75)
Disease:Hair shaft blebbing
Wild allele/target (5'->3'): AGAUCAAGACUCUGAACAACAA
Mutant allele/target (5'->3'):AGAUCAAGACUCUGAAC---AA
Target Mutation: c.545_547del,p.N159del
Wild siRNA (As strand):UCUAGUUCUGAGACUUGUUGUU
ASP-siRNA (As strand):UCUAGUUCUGAGACUUG---UU
Mismatch position in siRNA: del_18A,19A,20C,
Mutant allele (5'->3'): AGAUCAAGACUCUGAAC---AA
ASP-siRNA (As strand):UCUAGUUCUGAGACUUG---UU
ASP-siRNA efficacy for mutant allele:70
Wild allele (5'->3'): AGAUCAAGACUCUGAACAACAA
ASP-siRNA (As strand):UCUAGUUCUGAGACUUG---UU
ASP-siRNA efficacy for wild allele:52
Percentage discrimination for ASP-siRNA:18
Wild allele (5'->3'): AGAUCAAGACUCUGAACAACAA
Wild siRNA (As strand):UCUAGUUCUGAGACUUGUUGUU
Wild sirna efficacy for wild allele:50
Wild allele (5'->3'): AGAUCAAGACUCUGAACAACAA
ASP-siRNA (As strand):UCUAGUUCUGAGACUUG---UU
Wild sirna efficacy for mutant allele:42
Percentage discrimination for wild siRNA:8
Concentration:15 nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: O95678
Target sequence: ACGGGAGCAGATCAAGACTCTGAACAACAAGTTCGCCTCCTTC
Genbank Accesion: NM_004693.2
Cell-line: Primary keratinocytes
Experimental technique used: qRT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Thermo Fisher
Post-transfection duration: 48 hours
OMID ID: NA
KEGG disease ID:NA
Reference:26763422

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