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siRNA Id: | aspsirna3222
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siRNA Name: | siN159D-4
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Gene: | Keratin 75 (KRT75) |
Disease: | Hair shaft blebbing |
Wild allele/target (5'->3'): | GAUCAAGACUCUGAACAACAAG
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Mutant allele/target (5'->3'): | GAUCAAGACUCUGAAC---AAG
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Target Mutation: | c.545_547del,p.N159del
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Wild siRNA (As strand): | CUAGUUCUGAGACUUGUUGUUC
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ASP-siRNA (As strand): | CUAGUUCUGAGACUUG---UUC
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Mismatch position in siRNA: | del_17A,18A,19C, |
Mutant allele (5'->3'): | GAUCAAGACUCUGAAC---AAG
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ASP-siRNA (As strand): | CUAGUUCUGAGACUUG---UUC
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ASP-siRNA efficacy for mutant allele: | 54
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Wild allele (5'->3'): | GAUCAAGACUCUGAACAACAAG
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ASP-siRNA (As strand): | CUAGUUCUGAGACUUG---UUC
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ASP-siRNA efficacy for wild allele: | 0
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Percentage discrimination for ASP-siRNA: | 54
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Wild allele (5'->3'): | GAUCAAGACUCUGAACAACAAG
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Wild siRNA (As strand): | CUAGUUCUGAGACUUGUUGUUC
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Wild sirna efficacy for wild allele: | 50
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Wild allele (5'->3'): | GAUCAAGACUCUGAACAACAAG
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ASP-siRNA (As strand): | CUAGUUCUGAGACUUG---UUC
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Wild sirna efficacy for mutant allele: | 42
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Percentage discrimination for wild siRNA: | 8
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Concentration: | 15 nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | O95678 |
Target sequence: | ACGGGAGCAGATCAAGACTCTGAACAACAAGTTCGCCTCCTTC |
Genbank Accesion: | NM_004693.2 |
Cell-line: | Primary keratinocytes |
Experimental technique used: | qRT-PCR |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Thermo Fisher |
Post-transfection duration: | 48 hours |
OMID ID: | NA |
KEGG disease ID: | NA |
Reference: | 26763422 |