aspsirna3227 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3227
siRNA Name: siN159D-9
Gene:Keratin 75 (KRT75)
Disease:Hair shaft blebbing
Wild allele/target (5'->3'): AGACUCUGAACAACAAGUUCGC
Mutant allele/target (5'->3'):AGACUCUGAAC---AAGUUCGC
Target Mutation: c.545_547del,p.N159del
Wild siRNA (As strand):UCUGAGACUUGUUGUUCAAGCG
ASP-siRNA (As strand):UCUGAGACUUG---UUCAAGCG
Mismatch position in siRNA: del_12A,13A,14C,
Mutant allele (5'->3'): AGACUCUGAAC---AAGUUCGC
ASP-siRNA (As strand):UCUGAGACUUG---UUCAAGCG
ASP-siRNA efficacy for mutant allele:13
Wild allele (5'->3'): AGACUCUGAACAACAAGUUCGC
ASP-siRNA (As strand):UCUGAGACUUG---UUCAAGCG
ASP-siRNA efficacy for wild allele:57
Percentage discrimination for ASP-siRNA:-44
Wild allele (5'->3'): AGACUCUGAACAACAAGUUCGC
Wild siRNA (As strand):UCUGAGACUUGUUGUUCAAGCG
Wild sirna efficacy for wild allele:50
Wild allele (5'->3'): AGACUCUGAACAACAAGUUCGC
ASP-siRNA (As strand):UCUGAGACUUG---UUCAAGCG
Wild sirna efficacy for mutant allele:42
Percentage discrimination for wild siRNA:8
Concentration:15 nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: O95678
Target sequence: ACGGGAGCAGATCAAGACTCTGAACAACAAGTTCGCCTCCTTC
Genbank Accesion: NM_004693.2
Cell-line: Primary keratinocytes
Experimental technique used: qRT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Thermo Fisher
Post-transfection duration: 48 hours
OMID ID: NA
KEGG disease ID:NA
Reference:26763422

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