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siRNA Id: | aspsirna3257
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siRNA Name: | lv-shRNA167delinsWL-8
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| Gene: | keratin 9 (KRT9) |
| Disease: | Epidermolytic palmoplantar keratoderma (EPPK) |
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Wild allele/target (5'->3'): | GGCCUCUUACUUGGAU
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Mutant allele/target (5'->3'): | GGCCUCUUGGCUCUUGGAU
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Target Mutation: | c.500delAinsGGCT,p.Tyr167delinsTrpLeu
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Wild siRNA (As strand): | CCGGAGAAUGAACCUA
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ASP-siRNA (As strand): | CCGGAGAACCGAGAACCUA
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Mismatch position in siRNA: | delAinsGGCT |
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Mutant allele (5'->3'): | GGCCUCUUGGCUCUUGGAU
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ASP-siRNA (As strand): | CCGGAGAACCGAGAACCUA
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ASP-siRNA efficacy for mutant allele: | 80
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Wild allele (5'->3'): | GGCCUCUUACUUGGAU
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ASP-siRNA (As strand): | CCGGAGAACCGAGAACCUA
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ASP-siRNA efficacy for wild allele: | 2
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Percentage discrimination for ASP-siRNA: | 78
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Wild allele (5'->3'): | GGCCUCUUACUUGGAU
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Wild siRNA (As strand): | CCGGAGAAUGAACCUA
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Wild sirna efficacy for wild allele: | 40
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Wild allele (5'->3'): | GGCCUCUUACUUGGAU
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ASP-siRNA (As strand): | CCGGAGAACCGAGAACCUA
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Wild sirna efficacy for mutant allele: | 30
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Percentage discrimination for wild siRNA: | 10
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| Concentration: | 0 - 6.25 nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P35527 |
| Target sequence: | CTCGGCTGGCCTCTTACTTGGATAAGGTGCAGGCT |
| Genbank Accesion: | NM_000226.3 |
| Cell-line: | HaCaT |
| Experimental technique used: | Quantitative immunoblotting |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Genechem |
| Post-transfection duration: | 48 hours |
| OMID ID: | 144200 |
| KEGG disease ID: | H00722 |
| Reference: | 27003758 |
