| |
|---|
|
siRNA Id: | aspsirna3318
|
|
siRNA Name: | miSNP67T-17
|
| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
|
Wild allele/target (5'->3'): | UCUGCGCCCUUGUGCCCUCCA
|
|
Mutant allele/target (5'->3'): | UCUGUGCCCUUGUGCCCUCCA
|
|
Target Mutation: | NM_002111.6:c.9810C>T
|
|
Wild siRNA (As strand): | AGACGCGGGAACACGGGAGGU
|
|
ASP-siRNA (As strand): | AGACACGGGAACACGGGAGGU
|
|
Mismatch position in siRNA: | A5G |
|
Mutant allele (5'->3'): | UCUGUGCCCUUGUGCCCUCCA
|
|
ASP-siRNA (As strand): | AGACACGGGAACACGGGAGGU
|
|
ASP-siRNA efficacy for mutant allele: | 60
|
|
Wild allele (5'->3'): | UCUGCGCCCUUGUGCCCUCCA
|
|
ASP-siRNA (As strand): | AGACACGGGAACACGGGAGGU
|
|
ASP-siRNA efficacy for wild allele: | 52
|
|
Percentage discrimination for ASP-siRNA: | 8
|
|
Wild allele (5'->3'): | UCUGCGCCCUUGUGCCCUCCA
|
|
Wild siRNA (As strand): | AGACGCGGGAACACGGGAGGU
|
|
Wild sirna efficacy for wild allele: | 55
|
|
Wild allele (5'->3'): | UCUGCGCCCUUGUGCCCUCCA
|
|
ASP-siRNA (As strand): | AGACACGGGAACACGGGAGGU
|
|
Wild sirna efficacy for mutant allele: | 100
|
|
Percentage discrimination for wild siRNA: | -45
|
| Concentration: | 50 ng |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | AGGCGGCAGCTGGGGCCGGAGCCTTTGGAAGTCTGCGCCCTTG |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | HEK293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 27003755 |
