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siRNA Id: | aspsirna3353
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siRNA Name: | miSNP50T-18-11mm
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| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
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Wild allele/target (5'->3'): | AUCUACUGUGUGCACUUCAUC
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Mutant allele/target (5'->3'): | AUCUACUGUGGGCACUUCAUC
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Target Mutation: | NM_002111.7:c.6931T>C
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Wild siRNA (As strand): | UAGAUGACACACGUGAAGUAG
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ASP-siRNA (As strand): | UAGAUGACACCCGUGAAGUAG
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Mismatch position in siRNA: | A4G,C11A |
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Mutant allele (5'->3'): | AUCUACUGUGGGCACUUCAUC
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ASP-siRNA (As strand): | UAGAUGACACCCGUGAAGUAG
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ASP-siRNA efficacy for mutant allele: | 75
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Wild allele (5'->3'): | AUCUACUGUGUGCACUUCAUC
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ASP-siRNA (As strand): | UAGAUGACACCCGUGAAGUAG
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ASP-siRNA efficacy for wild allele: | 45
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Percentage discrimination for ASP-siRNA: | 30
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Wild allele (5'->3'): | AUCUACUGUGUGCACUUCAUC
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Wild siRNA (As strand): | UAGAUGACACACGUGAAGUAG
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Wild sirna efficacy for wild allele: | 60
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Wild allele (5'->3'): | AUCUACUGUGUGCACUUCAUC
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ASP-siRNA (As strand): | UAGAUGACACCCGUGAAGUAG
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Wild sirna efficacy for mutant allele: | 90
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Percentage discrimination for wild siRNA: | -30
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| Concentration: | 50 ng |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | TTGTGACCCACGCCTGCTCCCTCATCTACTGTGTGCACTTCAT |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | HEK293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 27003755 |
