aspsirna3483 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3483
siRNA Name: sh67C
Gene:Hungtintin (HTT)
Disease:Huntington disease (HD)
Wild allele/target (5'->3'): GGAAGUCUGAGCCCUUGUG
Mutant allele/target (5'->3'):GGAAGUCUGCGCCCUUGUG
Target Mutation: NM_002111.6:c.9810C>T
Wild siRNA (As strand):CCUUCAGACUCGGGAACAC
ASP-siRNA (As strand):CCUUCAGACGCGGGAACAC
Mismatch position in siRNA: G10U
Mutant allele (5'->3'): GGAAGUCUGCGCCCUUGUG
ASP-siRNA (As strand):CCUUCAGACGCGGGAACAC
ASP-siRNA efficacy for mutant allele:85
Wild allele (5'->3'): GGAAGUCUGAGCCCUUGUG
ASP-siRNA (As strand):CCUUCAGACGCGGGAACAC
ASP-siRNA efficacy for wild allele:55
Percentage discrimination for ASP-siRNA:30
Wild allele (5'->3'): GGAAGUCUGAGCCCUUGUG
Wild siRNA (As strand):CCUUCAGACUCGGGAACAC
Wild sirna efficacy for wild allele:76
Wild allele (5'->3'): GGAAGUCUGAGCCCUUGUG
ASP-siRNA (As strand):CCUUCAGACGCGGGAACAC
Wild sirna efficacy for mutant allele:45
Percentage discrimination for wild siRNA:31
Concentration:6nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P42858
Target sequence: AGCTTTGATGGATTCTAA
Genbank Accesion: NM_002111.7
Cell-line: BACHD mice
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 8 weeks
OMID ID: 143100
KEGG disease ID:H00059
Reference:24926995

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