aspsirna3486 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3486
siRNA Name: sh39Ap16
Gene:Hungtintin (HTT)
Disease:Huntington disease (HD)
Wild allele/target (5'->3'): GUACUUCAACGCUAGAA
Mutant allele/target (5'->3'):GUAAUUCAACGCUAGAA
Target Mutation: NM_002111.7:c.5165C>A
Wild siRNA (As strand):CAUGAAGUUGCGAUCUU
ASP-siRNA (As strand):CAUUAAGUUGCGAUCUU
Mismatch position in siRNA: U16G
Mutant allele (5'->3'): GUAAUUCAACGCUAGAA
ASP-siRNA (As strand):CAUUAAGUUGCGAUCUU
ASP-siRNA efficacy for mutant allele:98
Wild allele (5'->3'): GUACUUCAACGCUAGAA
ASP-siRNA (As strand):CAUUAAGUUGCGAUCUU
ASP-siRNA efficacy for wild allele:40
Percentage discrimination for ASP-siRNA:58
Wild allele (5'->3'): GUACUUCAACGCUAGAA
Wild siRNA (As strand):CAUGAAGUUGCGAUCUU
Wild sirna efficacy for wild allele:88
Wild allele (5'->3'): GUACUUCAACGCUAGAA
ASP-siRNA (As strand):CAUUAAGUUGCGAUCUU
Wild sirna efficacy for mutant allele:15
Percentage discrimination for wild siRNA:73
Concentration:6nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P42858
Target sequence: AGCTTTGATGGATTCTAA
Genbank Accesion: NM_002111.7
Cell-line: HD-human Embryonic Stem cells(hESCs)
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 8 weeks
OMID ID: 143100
KEGG disease ID:H00059
Reference:24926995

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