| |
|---|
|
siRNA Id: | aspsirna3505
|
|
siRNA Name: | siPrnp178(A8)
|
| Gene: | Human prion protein (PRNP) |
| Disease: | Prion disease/Creutzfeldt-Jakob disease |
|
Wild allele/target (5'->3'): | UGUGCACGACUGCGUCAAU
|
|
Mutant allele/target (5'->3'): | UGUGCACAACUGCGUCAAU
|
|
Target Mutation: | c.532G>A,p.Asp178Asn
|
|
Wild siRNA (As strand): | ACACGUGCUGACGCAGUUA
|
|
ASP-siRNA (As strand): | ACACGUGUUGACGCAGUUA
|
|
Mismatch position in siRNA: | U12C |
|
Mutant allele (5'->3'): | UGUGCACAACUGCGUCAAU
|
|
ASP-siRNA (As strand): | ACACGUGUUGACGCAGUUA
|
|
ASP-siRNA efficacy for mutant allele: | 88
|
|
Wild allele (5'->3'): | UGUGCACGACUGCGUCAAU
|
|
ASP-siRNA (As strand): | ACACGUGUUGACGCAGUUA
|
|
ASP-siRNA efficacy for wild allele: | 70
|
|
Percentage discrimination for ASP-siRNA: | 18
|
|
Wild allele (5'->3'): | UGUGCACGACUGCGUCAAU
|
|
Wild siRNA (As strand): | ACACGUGCUGACGCAGUUA
|
|
Wild sirna efficacy for wild allele: | 90
|
|
Wild allele (5'->3'): | UGUGCACGACUGCGUCAAU
|
|
ASP-siRNA (As strand): | ACACGUGUUGACGCAGUUA
|
|
Wild sirna efficacy for mutant allele: | 20
|
|
Percentage discrimination for wild siRNA: | 70
|
| Concentration: | 40nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P04156 |
| Target sequence: | AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA |
| Genbank Accesion: | NM_000311.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 176640 |
| KEGG disease ID: | H00061 |
| Reference: | 18493311 |
