| |
|---|
|
siRNA Id: | aspsirna3510
|
|
siRNA Name: | K12-Arg135Thr-2
|
| Gene: | Keratin 12 (KRT12) |
| Disease: | Meesmann epithelial corneal dystrophy (MECD) |
|
Wild allele/target (5'->3'): | GCAAAAUCUUAAUGAUAGA
|
|
Mutant allele/target (5'->3'): | GCAAAAUCUUAAUGAUACA
|
|
Target Mutation: | c.404G>C,p.Arg135Thr
|
|
Wild siRNA (As strand): | CGUUUUAGAAUUACUAUCU
|
|
ASP-siRNA (As strand): | CGUUUUAGAAUUACUAUGU
|
|
Mismatch position in siRNA: | G2C |
|
Mutant allele (5'->3'): | GCAAAAUCUUAAUGAUACA
|
|
ASP-siRNA (As strand): | CGUUUUAGAAUUACUAUGU
|
|
ASP-siRNA efficacy for mutant allele: | 35
|
|
Wild allele (5'->3'): | GCAAAAUCUUAAUGAUAGA
|
|
ASP-siRNA (As strand): | CGUUUUAGAAUUACUAUGU
|
|
ASP-siRNA efficacy for wild allele: | 20
|
|
Percentage discrimination for ASP-siRNA: | 15
|
|
Wild allele (5'->3'): | GCAAAAUCUUAAUGAUAGA
|
|
Wild siRNA (As strand): | CGUUUUAGAAUUACUAUCU
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | GCAAAAUCUUAAUGAUAGA
|
|
ASP-siRNA (As strand): | CGUUUUAGAAUUACUAUGU
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 0.25nM, 5nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q99456 |
| Target sequence: | GAAAAAGAAACTATGCAAAATCTTAATGATAG |
| Genbank Accesion: | NM_000223.3 |
| Cell-line: | HaCaT |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | MWG Biotech AG |
| Post-transfection duration: | 72 hours |
| OMID ID: | 122100 |
| KEGG disease ID: | H00726 |
| Reference: | 23233254 |
