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siRNA Id: | aspsirna3527
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siRNA Name: | K12-Arg135Thr-19
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| Gene: | Keratin 12 (KRT12) |
| Disease: | Meesmann epithelial corneal dystrophy (MECD) |
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Wild allele/target (5'->3'): | GAUUAGCUUCCUACCUGGA
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Mutant allele/target (5'->3'): | CAUUAGCUUCCUACCUGGA
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Target Mutation: | c.404G>C,p.Arg135Thr
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Wild siRNA (As strand): | CUAAUCGAAGGAUGGACCU
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ASP-siRNA (As strand): | GUAAUCGAAGGAUGGACCU
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Mismatch position in siRNA: | G19C |
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Mutant allele (5'->3'): | CAUUAGCUUCCUACCUGGA
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ASP-siRNA (As strand): | GUAAUCGAAGGAUGGACCU
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ASP-siRNA efficacy for mutant allele: | 36
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Wild allele (5'->3'): | GAUUAGCUUCCUACCUGGA
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ASP-siRNA (As strand): | GUAAUCGAAGGAUGGACCU
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 6
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Wild allele (5'->3'): | GAUUAGCUUCCUACCUGGA
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Wild siRNA (As strand): | CUAAUCGAAGGAUGGACCU
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GAUUAGCUUCCUACCUGGA
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ASP-siRNA (As strand): | GUAAUCGAAGGAUGGACCU
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 0.25nM, 5nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q99456 |
| Target sequence: | GAAAAAGAAACTATGCAAAATCTTAATGATAG |
| Genbank Accesion: | NM_000223.3 |
| Cell-line: | HaCaT |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | MWG Biotech AG |
| Post-transfection duration: | 72 hours |
| OMID ID: | 122100 |
| KEGG disease ID: | H00726 |
| Reference: | 23233254 |
