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siRNA Id: | aspsirna3539
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siRNA Name: | siA9/C12
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| Gene: | Microtubule-associated protein tau (MAPT) |
| Disease: | Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) |
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Wild allele/target (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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Mutant allele/target (5'->3'): | GUGGCCAGAUGCAAGUAAAAUC
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Target Mutation: | c.1960G>A,p.Val654Met
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Wild siRNA (As strand): | CACCGGUCCACCUUCAUUUUAG
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ASP-siRNA (As strand): | CACCGGUCUACGUUCAUUUUAG
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Mismatch position in siRNA: | U14C,G11C |
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Mutant allele (5'->3'): | GUGGCCAGAUGCAAGUAAAAUC
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ASP-siRNA (As strand): | CACCGGUCUACGUUCAUUUUAG
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ASP-siRNA efficacy for mutant allele: | 28
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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ASP-siRNA (As strand): | CACCGGUCUACGUUCAUUUUAG
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ASP-siRNA efficacy for wild allele: | 55
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Percentage discrimination for ASP-siRNA: | -27
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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Wild siRNA (As strand): | CACCGGUCCACCUUCAUUUUAG
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Wild sirna efficacy for wild allele: | 49
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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ASP-siRNA (As strand): | CACCGGUCUACGUUCAUUUUAG
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Wild sirna efficacy for mutant allele: | 20
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Percentage discrimination for wild siRNA: | 29
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| Concentration: | 2.5mg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P10636 |
| Target sequence: | GUGGCCAGGUGGAAGUAAAAUC |
| Genbank Accesion: | NM_016835.4 |
| Cell-line: | Cos-7 |
| Experimental technique used: | Fluorescence microscopy |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 24 hours |
| OMID ID: | 600274 |
| KEGG disease ID: | H00078 |
| Reference: | US20080176812A1 |
