aspsirna3551 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna3551
siRNA Name: siA9/C12
Gene:Microtubule-associated protein tau (MAPT)
Disease:Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17)
Wild allele/target (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
Mutant allele/target (5'->3'):GUGGCCAGAUGCAAGUAAAAUC
Target Mutation: c.1960G>A,p.Val654Met
Wild siRNA (As strand):CACCGGUCCACCUUCAUUUUAG
ASP-siRNA (As strand):CACCGGUCUACGUUCAUUUUAG
Mismatch position in siRNA: U14C,G11C
Mutant allele (5'->3'): GUGGCCAGAUGCAAGUAAAAUC
ASP-siRNA (As strand):CACCGGUCUACGUUCAUUUUAG
ASP-siRNA efficacy for mutant allele:28
Wild allele (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
ASP-siRNA (As strand):CACCGGUCUACGUUCAUUUUAG
ASP-siRNA efficacy for wild allele:55
Percentage discrimination for ASP-siRNA:-27
Wild allele (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
Wild siRNA (As strand):CACCGGUCCACCUUCAUUUUAG
Wild sirna efficacy for wild allele:49
Wild allele (5'->3'): GUGGCCAGGUGGAAGUAAAAUC
ASP-siRNA (As strand):CACCGGUCUACGUUCAUUUUAG
Wild sirna efficacy for mutant allele:20
Percentage discrimination for wild siRNA:29
Concentration:2.5mg
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P10636
Target sequence: GUGGCCAGGUGGAAGUAAAAUC
Genbank Accesion: NM_016835.4
Cell-line: Cos-7
Experimental technique used: Western blot
Transfection method: Lipofectamine Plus
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 24 hours
OMID ID: 600274
KEGG disease ID:H00078
Reference:US20080176812A1

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