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siRNA Id: | aspsirna3558
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siRNA Name: | p9mut
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| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
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Wild allele/target (5'->3'): | UGGAGACUUGGGCAAUGUG
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Mutant allele/target (5'->3'): | UGGAGACUUGCGCAAUGUG
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Target Mutation: | c.256G>C,p.Gly86Arg
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Wild siRNA (As strand): | ACCUCUGAACCCGUUACAC
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ASP-siRNA (As strand): | ACCUCUGAACGCGUUACAC
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Mismatch position in siRNA: | G9C |
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Mutant allele (5'->3'): | UGGAGACUUGCGCAAUGUG
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ASP-siRNA (As strand): | ACCUCUGAACGCGUUACAC
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ASP-siRNA efficacy for mutant allele: | 80
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Wild allele (5'->3'): | UGGAGACUUGGGCAAUGUG
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ASP-siRNA (As strand): | ACCUCUGAACGCGUUACAC
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ASP-siRNA efficacy for wild allele: | 10
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Percentage discrimination for ASP-siRNA: | 70
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Wild allele (5'->3'): | UGGAGACUUGGGCAAUGUG
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Wild siRNA (As strand): | ACCUCUGAACCCGUUACAC
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Wild sirna efficacy for wild allele: | Lowest effective
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Wild allele (5'->3'): | UGGAGACUUGGGCAAUGUG
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ASP-siRNA (As strand): | ACCUCUGAACGCGUUACAC
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Wild sirna efficacy for mutant allele: | 93
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Percentage discrimination for wild siRNA: | High discrimination
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| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | GAGAGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAG |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | EP1567539B1 |
