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siRNA Id: | aspsirna3573
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siRNA Name: | A11
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| Gene: | Activin A type I receptor proteins (ACVR1) |
| Disease: | Fibrodysplasia Ossificans Progressiva (FOP) |
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Wild allele/target (5'->3'): | ACAGUGGCUCGCCAGAUUA
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Mutant allele/target (5'->3'): | ACAGUGGCUCACCAGAUUA
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Target Mutation: | c.617G>A,p.Arg206His
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Wild siRNA (As strand): | UGUCACCGAGCGGUCUAAU
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ASP-siRNA (As strand): | UGUCACCGAGUGGUCUAAU
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Mismatch position in siRNA: | U9C |
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Mutant allele (5'->3'): | ACAGUGGCUCACCAGAUUA
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ASP-siRNA (As strand): | UGUCACCGAGUGGUCUAAU
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ASP-siRNA efficacy for mutant allele: | 50
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Wild allele (5'->3'): | ACAGUGGCUCGCCAGAUUA
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ASP-siRNA (As strand): | UGUCACCGAGUGGUCUAAU
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ASP-siRNA efficacy for wild allele: | 20
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Percentage discrimination for ASP-siRNA: | 30
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Wild allele (5'->3'): | ACAGUGGCUCGCCAGAUUA
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Wild siRNA (As strand): | UGUCACCGAGCGGUCUAAU
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Wild sirna efficacy for wild allele: | 45
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Wild allele (5'->3'): | ACAGUGGCUCGCCAGAUUA
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ASP-siRNA (As strand): | UGUCACCGAGUGGUCUAAU
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Wild sirna efficacy for mutant allele: | 40
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Percentage discrimination for wild siRNA: | 5
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| Concentration: | 10nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | |
| Target sequence: | AACAGTGGCTCGCCAGATTACACTGTTGGAGT |
| Cell-line: | FOP SHED cells |
| Experimental technique used: | Immunopblotting |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 135100 |
| KEGG disease ID: | H00430 |
| Reference: | US8859752B2 |
