| |
|---|
|
siRNA Id: | aspsirna3574
|
|
siRNA Name: | A12
|
| Gene: | Activin A type I receptor proteins (ACVR1) |
| Disease: | Fibrodysplasia Ossificans Progressiva (FOP) |
|
Wild allele/target (5'->3'): | AACAGUGGCUCGCCAGAUU
|
|
Mutant allele/target (5'->3'): | AACAGUGGCUCACCAGAUU
|
|
Target Mutation: | c.617G>A,p.Arg206His
|
|
Wild siRNA (As strand): | UUGUCACCGAGCGGUCUAA
|
|
ASP-siRNA (As strand): | UUGUCACCGAGUGGUCUAA
|
|
Mismatch position in siRNA: | U8C |
|
Mutant allele (5'->3'): | AACAGUGGCUCACCAGAUU
|
|
ASP-siRNA (As strand): | UUGUCACCGAGUGGUCUAA
|
|
ASP-siRNA efficacy for mutant allele: | 60
|
|
Wild allele (5'->3'): | AACAGUGGCUCGCCAGAUU
|
|
ASP-siRNA (As strand): | UUGUCACCGAGUGGUCUAA
|
|
ASP-siRNA efficacy for wild allele: | 39
|
|
Percentage discrimination for ASP-siRNA: | 21
|
|
Wild allele (5'->3'): | AACAGUGGCUCGCCAGAUU
|
|
Wild siRNA (As strand): | UUGUCACCGAGCGGUCUAA
|
|
Wild sirna efficacy for wild allele: | 45
|
|
Wild allele (5'->3'): | AACAGUGGCUCGCCAGAUU
|
|
ASP-siRNA (As strand): | UUGUCACCGAGUGGUCUAA
|
|
Wild sirna efficacy for mutant allele: | 40
|
|
Percentage discrimination for wild siRNA: | 5
|
| Concentration: | 10nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | |
| Target sequence: | AACAGTGGCTCGCCAGATTACACTGTTGGAGT |
| Cell-line: | FOP SHED cells |
| Experimental technique used: | Immunopblotting |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 135100 |
| KEGG disease ID: | H00430 |
| Reference: | US8859752B2 |
