| |
|---|
|
siRNA Id: | aspsirna3642
|
|
siRNA Name: | 66
|
| Gene: | Peptidylprolyl isomerase B (PPIB)/Cyclophilin B |
| Disease: | PPIB-Related Osteogenesis Imperfecta (Type IX) |
|
Wild allele/target (5'->3'): | UCAGAGAGAUCCUCAUAAA
|
|
Mutant allele/target (5'->3'): | UCAGAGAGAUCCUCAUAAA
|
|
Target Mutation: | NA
|
|
Wild siRNA (As strand): | AGUCUCUCUAGGAGUAUUU
|
|
ASP-siRNA (As strand): | AGACUCUCUAGGAGUAUUU
|
|
Mismatch position in siRNA: | A17U |
|
Mutant allele (5'->3'): | UCAGAGAGAUCCUCAUAAA
|
|
ASP-siRNA (As strand): | AGACUCUCUAGGAGUAUUU
|
|
ASP-siRNA efficacy for mutant allele: | 90
|
|
Wild allele (5'->3'): | UCAGAGAGAUCCUCAUAAA
|
|
ASP-siRNA (As strand): | AGACUCUCUAGGAGUAUUU
|
|
ASP-siRNA efficacy for wild allele: | 72
|
|
Percentage discrimination for ASP-siRNA: | 18
|
|
Wild allele (5'->3'): | UCAGAGAGAUCCUCAUAAA
|
|
Wild siRNA (As strand): | AGUCUCUCUAGGAGUAUUU
|
|
Wild sirna efficacy for wild allele: | 90
|
|
Wild allele (5'->3'): | UCAGAGAGAUCCUCAUAAA
|
|
ASP-siRNA (As strand): | AGACUCUCUAGGAGUAUUU
|
|
Wild sirna efficacy for mutant allele: | 72
|
|
Percentage discrimination for wild siRNA: | 18
|
| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P23284 |
| Target sequence: | CGCAGGCAAAGACACCAACGGCTCCCAGTTCTTCAT |
| Genbank Accesion: | NM_000942.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | 2' ACE-chemistry |
| Post-transfection duration: | 24 hours |
| OMID ID: | 166200 |
| KEGG disease ID: | H00506 |
| Reference: | 16489337 |
