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siRNA Id: | aspsirna3755
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siRNA Name: | 179
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| Gene: | Peptidylprolyl isomerase B (PPIB)/Cyclophilin B |
| Disease: | PPIB-Related Osteogenesis Imperfecta (Type IX) |
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Wild allele/target (5'->3'): | AAAGUUGCGCGGAGGAGUU
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Mutant allele/target (5'->3'): | AAAGUUGCGCGGAGGAGUU
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Target Mutation: | NA
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Wild siRNA (As strand): | UUUCAACGCGCCUCCUCAA
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ASP-siRNA (As strand): | UUUCAACGCGCCACCUCAA
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Mismatch position in siRNA: | A7U |
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Mutant allele (5'->3'): | AAAGUUGCGCGGAGGAGUU
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ASP-siRNA (As strand): | UUUCAACGCGCCACCUCAA
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ASP-siRNA efficacy for mutant allele: | 90
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Wild allele (5'->3'): | AAAGUUGCGCGGAGGAGUU
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ASP-siRNA (As strand): | UUUCAACGCGCCACCUCAA
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ASP-siRNA efficacy for wild allele: | 48
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Percentage discrimination for ASP-siRNA: | 42
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Wild allele (5'->3'): | AAAGUUGCGCGGAGGAGUU
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Wild siRNA (As strand): | UUUCAACGCGCCUCCUCAA
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Wild sirna efficacy for wild allele: | 90
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Wild allele (5'->3'): | AAAGUUGCGCGGAGGAGUU
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ASP-siRNA (As strand): | UUUCAACGCGCCACCUCAA
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Wild sirna efficacy for mutant allele: | 48
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Percentage discrimination for wild siRNA: | 42
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| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P23284 |
| Target sequence: | CGCAGGCAAAGACACCAACGGCTCCCAGTTCTTCAT |
| Genbank Accesion: | NM_000942.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | 2' ACE-chemistry |
| Post-transfection duration: | 24 hours |
| OMID ID: | 166200 |
| KEGG disease ID: | H00506 |
| Reference: | 16489337 |
