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siRNA Id: | aspsirna3887
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siRNA Name: | siRNA-4_12U
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| Gene: | Transforming growth factor, beta receptor II (TGFBR2) |
| Disease: | NA |
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Wild allele/target (5'->3'): | GGUUAACAGCGAUCUGAUG
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Mutant allele/target (5'->3'): | GGUUAACGGCGAUCUGAUG
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Target Mutation: | NA
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Wild siRNA (As strand): | CCAAUUGUCGCUAGACUAC
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ASP-siRNA (As strand): | CCAAUUGUCGCUAGACUAC
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Mismatch position in siRNA: | U:G |
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Mutant allele (5'->3'): | GGUUAACGGCGAUCUGAUG
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ASP-siRNA (As strand): | CCAAUUGUCGCUAGACUAC
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ASP-siRNA efficacy for mutant allele: | 64
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Wild allele (5'->3'): | GGUUAACAGCGAUCUGAUG
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ASP-siRNA (As strand): | CCAAUUGUCGCUAGACUAC
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ASP-siRNA efficacy for wild allele: | 95
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Percentage discrimination for ASP-siRNA: | 31
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Wild allele (5'->3'): | GGUUAACAGCGAUCUGAUG
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Wild siRNA (As strand): | CCAAUUGUCGCUAGACUAC
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Wild sirna efficacy for wild allele: | 95
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Wild allele (5'->3'): | GGUUAACAGCGAUCUGAUG
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ASP-siRNA (As strand): | CCAAUUGUCGCUAGACUAC
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Wild sirna efficacy for mutant allele: | 45
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Percentage discrimination for wild siRNA: | 50
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| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P37173 |
| Target sequence: | CCGCACGTTCCCAAGTCGGTTAACAGCGATCTGATG |
| Genbank Accesion: | NM_031132.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 19815667 |
