| |
|---|
|
siRNA Id: | aspsirna3897
|
|
siRNA Name: | siRNA-6_17U
|
| Gene: | Transforming growth factor, beta receptor II (TGFBR2) |
| Disease: | NA |
|
Wild allele/target (5'->3'): | GGAGUGUAACGAUUACAUC
|
|
Mutant allele/target (5'->3'): | GGUGUGUAACGAUUACAUC
|
|
Target Mutation: | NA
|
|
Wild siRNA (As strand): | CCUCACAUUGCUAAUGUAG
|
|
ASP-siRNA (As strand): | CCUCACAUUGCUAAUGUAG
|
|
Mismatch position in siRNA: | U:U |
|
Mutant allele (5'->3'): | GGUGUGUAACGAUUACAUC
|
|
ASP-siRNA (As strand): | CCUCACAUUGCUAAUGUAG
|
|
ASP-siRNA efficacy for mutant allele: | 90
|
|
Wild allele (5'->3'): | GGAGUGUAACGAUUACAUC
|
|
ASP-siRNA (As strand): | CCUCACAUUGCUAAUGUAG
|
|
ASP-siRNA efficacy for wild allele: | 95
|
|
Percentage discrimination for ASP-siRNA: | 5
|
|
Wild allele (5'->3'): | GGAGUGUAACGAUUACAUC
|
|
Wild siRNA (As strand): | CCUCACAUUGCUAAUGUAG
|
|
Wild sirna efficacy for wild allele: | 98
|
|
Wild allele (5'->3'): | GGAGUGUAACGAUUACAUC
|
|
ASP-siRNA (As strand): | CCUCACAUUGCUAAUGUAG
|
|
Wild sirna efficacy for mutant allele: | 75
|
|
Percentage discrimination for wild siRNA: | 23
|
| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P37173 |
| Target sequence: | ATGTGCTCCTGTAACACAGAGGAGTGTAACGATTACATC |
| Genbank Accesion: | NM_031132.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 19815667 |
