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siRNA Id: | aspsirna3920
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siRNA Name: | siRNA-13_17U
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| Gene: | Sphingosine 1-phosphate receptor1(S1PR1) |
| Disease: | NA |
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Wild allele/target (5'->3'): | GUAACUGAAGGCUCGCUCA
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Mutant allele/target (5'->3'): | GUCACUGAAGGCUCGCUCA
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Target Mutation: | NA
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Wild siRNA (As strand): | CAUUGACUUCCGAGCGAGU
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ASP-siRNA (As strand): | CAUUGACUUCCGAGCGAGU
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Mismatch position in siRNA: | U:C |
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Mutant allele (5'->3'): | GUCACUGAAGGCUCGCUCA
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ASP-siRNA (As strand): | CAUUGACUUCCGAGCGAGU
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ASP-siRNA efficacy for mutant allele: | 75
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Wild allele (5'->3'): | GUAACUGAAGGCUCGCUCA
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ASP-siRNA (As strand): | CAUUGACUUCCGAGCGAGU
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ASP-siRNA efficacy for wild allele: | 95
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Percentage discrimination for ASP-siRNA: | 20
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Wild allele (5'->3'): | GUAACUGAAGGCUCGCUCA
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Wild siRNA (As strand): | CAUUGACUUCCGAGCGAGU
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Wild sirna efficacy for wild allele: | 97
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Wild allele (5'->3'): | GUAACUGAAGGCUCGCUCA
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ASP-siRNA (As strand): | CAUUGACUUCCGAGCGAGU
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Wild sirna efficacy for mutant allele: | 70
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Percentage discrimination for wild siRNA: | 27
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| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P21453 |
| Target sequence: | CTGTAACTGAAGGCTCGCTCAACCTCGCCCT |
| Genbank Accesion: | NM_017301.2 |
| Cell-line: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 19815667 |
