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siRNA Id: | aspsirna3951
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siRNA Name: | siRNA-21_4G
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| Gene: | Polyribonucleotide nucleotidyltransferase 1 (PNPT1) |
| Disease: | NA |
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Wild allele/target (5'->3'): | GGCUCAAGUGAUUCUCCUG
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Mutant allele/target (5'->3'): | GGCUCAAGUGAUUCUGCUG
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Target Mutation: | NA
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Wild siRNA (As strand): | CCGAGUUCACUAAGAGGAC
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ASP-siRNA (As strand): | CCGAGUUCACUAAGAGGAC
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Mismatch position in siRNA: | G:G |
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Mutant allele (5'->3'): | GGCUCAAGUGAUUCUGCUG
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ASP-siRNA (As strand): | CCGAGUUCACUAAGAGGAC
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ASP-siRNA efficacy for mutant allele: | 70
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Wild allele (5'->3'): | GGCUCAAGUGAUUCUCCUG
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ASP-siRNA (As strand): | CCGAGUUCACUAAGAGGAC
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ASP-siRNA efficacy for wild allele: | 96
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Percentage discrimination for ASP-siRNA: | 26
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Wild allele (5'->3'): | GGCUCAAGUGAUUCUCCUG
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Wild siRNA (As strand): | CCGAGUUCACUAAGAGGAC
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Wild sirna efficacy for wild allele: | 99
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Wild allele (5'->3'): | GGCUCAAGUGAUUCUCCUG
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ASP-siRNA (As strand): | CCGAGUUCACUAAGAGGAC
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Wild sirna efficacy for mutant allele: | 60
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Percentage discrimination for wild siRNA: | 39
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| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q8TCS8 |
| Target sequence: | NA |
| Genbank Accesion: | NM_033109.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 19815667 |
