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siRNA Id: | aspsirna3977
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siRNA Name: | siRNA-25_4C
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| Gene: | Collagen type I alpha 1 (COL1A2) |
| Disease: | NA |
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Wild allele/target (5'->3'): | GAACUUUGCUGCUCAGUAU
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Mutant allele/target (5'->3'): | GAACUUUGCUGCUCAAUAU
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Target Mutation: | NA
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Wild siRNA (As strand): | CUUGAAACGACGAGUCAUA
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ASP-siRNA (As strand): | CUUGAAACGACGAGUCAUA
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Mismatch position in siRNA: | C:A |
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Mutant allele (5'->3'): | GAACUUUGCUGCUCAAUAU
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ASP-siRNA (As strand): | CUUGAAACGACGAGUCAUA
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ASP-siRNA efficacy for mutant allele: | 90
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Wild allele (5'->3'): | GAACUUUGCUGCUCAGUAU
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ASP-siRNA (As strand): | CUUGAAACGACGAGUCAUA
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ASP-siRNA efficacy for wild allele: | 97
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Percentage discrimination for ASP-siRNA: | 7
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Wild allele (5'->3'): | GAACUUUGCUGCUCAGUAU
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Wild siRNA (As strand): | CUUGAAACGACGAGUCAUA
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Wild sirna efficacy for wild allele: | 99
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Wild allele (5'->3'): | GAACUUUGCUGCUCAGUAU
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ASP-siRNA (As strand): | CUUGAAACGACGAGUCAUA
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Wild sirna efficacy for mutant allele: | 70
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Percentage discrimination for wild siRNA: | 29
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| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P02452 |
| Target sequence: | CTGGTCTTACTGGGAACTTTGCTGCTCAGTATTC |
| Genbank Accesion: | NM_053356.1 |
| Cell-line: | HEK293 |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | TriZol reagent |
| siRNA expression method: | Genepharma, Proligo Sigma |
| Post-transfection duration: | 24 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 19815667 |
