aspsirna0045 details

''''
ASPsiRNA information siRNA Id:aspsirna0045
siRNA Name: p11Gmm10U
Mismatch information Wild siRNA (As strand 3'->5'):CCUGUCAUGAAGUUGCGAU
ASP-siRNA (As strand 3'->5'):CCUGUCAUGUAGUUGCGAU
Mismatch position in siRNA: U10A,G11U
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):GGACAGUACAUCAACGCUA
Wild allele (5'->3'):GGACAGUACUUCAACGCUA
Mutant allele (5'->3'):GGACAGUACAUCAACGCUA
Position of siRNA on target gene: 5302-5320
Respective Gene/Protein Resources GenBank Accession:NM_002111.6
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:Q96CV9
HUGO ID:4851
Reference SNp(RefSNP): rs363125
Disease/Mutation information Target Mutation: c.5165C>A,c.52CAG(36_39)
Matation type/variant: Single nucleotide variant/Missense variant/Microsatellite
Mutation at gene level: NG_009378.1:g.118146C>A,NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H01243
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): GGACAGUACAUCAACGCUA
ASP-siRNA (As strand 3'->5'):CCUGUCAUGUAGUUGCGAU
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): GGACAGUACUUCAACGCUA
ASP-siRNA (As strand 3'->5'):CCUGUCAUGUAGUUGCGAU
Percentage efficacy of ASP-siRNA for wild allele:60
Relative difference:30
Wild siRNA details Wild allele (5'->3'): GGACAGUACUUCAACGCUA
Wild siRNA (As strand 3'->5'):CCUGUCAUGAAGUUGCGAU
Percentage efficacy of wild siRNA for wild allele:80
Wild allele (5'->3'): GGACAGUACUUCAACGCUA
ASP-siRNA (As strand 3'->5'):CCUGUCAUGUAGUUGCGAU
Percentage efficacy of Wild sirna for mutant allele:30
Relative difference :50
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:10pmol
Reference:19289118
Delivery method:Transfection
Article title:A majority of Huntington's disease patients may be treatable by individualized allele-specific RNA interference.
Authors:Lombardi MS, Jaspers L, Spronkmans C, Gellera C, Taroni F, Di Maria E, Donato SD, Kaemmerer WF.
Journal Reference:Exp Neurol. 2009 Jun;217(2):312-9. doi: 10.1016/j.expneurol.2009.03.004. Epub 2009 Mar 13.