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| ASPsiRNA information |
siRNA Id: | aspsirna0045
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siRNA Name: | p11Gmm10U
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CCUGUCAUGAAGUUGCGAU
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ASP-siRNA (As strand 3'->5'): | CCUGUCAUGUAGUUGCGAU
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Mismatch position in siRNA: | U10A,G11U |
| Gene Information |
Gene Name | Hungtintin (HTT) |
|
Target Sequence (5'->3'): | GGACAGUACAUCAACGCUA
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Wild allele (5'->3'): | GGACAGUACUUCAACGCUA
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Mutant allele (5'->3'): | GGACAGUACAUCAACGCUA
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Position of siRNA on target gene: | 5302-5320
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.6 |
| Cytogenic location: | 4p16.3 |
| Chromosomal coordinates: | 4:3,076,407-3,245,686 |
| UniProt ID: | Q96CV9 |
| HUGO ID: | 4851 |
|
Reference SNp(RefSNP): | rs363125 |
| Disease/Mutation information |
Target Mutation: | c.5165C>A,c.52CAG(36_39)
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Matation type/variant: | Single nucleotide variant/Missense variant/Microsatellite
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Mutation at gene level: | NG_009378.1:g.118146C>A,NG_009378.1:g.5197CAG(36_39)
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Pathogenic status of mutation: | Pathogenic
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Disease: | Huntington disease (HD) |
| Clinical Resources |
ClinVar ID: | 31916 |
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KEGG disease ID: | H01243 |
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OMIM ID: | 143100 |
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COSMIC: | HTT |
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DECIPHER: | HTT |
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GeneTests: | HTT |
| ASP siRNA details |
Mutant allele (5'->3'): | GGACAGUACAUCAACGCUA
|
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ASP-siRNA (As strand 3'->5'): | CCUGUCAUGUAGUUGCGAU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 90
|
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Wild allele (5'->3'): | GGACAGUACUUCAACGCUA
|
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ASP-siRNA (As strand 3'->5'): | CCUGUCAUGUAGUUGCGAU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 60
|
|
Relative difference: | 30
|
| Wild siRNA details |
Wild allele (5'->3'): | GGACAGUACUUCAACGCUA
|
|
Wild siRNA (As strand 3'->5'): | CCUGUCAUGAAGUUGCGAU
|
|
Percentage efficacy of wild siRNA for wild allele: | 80
|
|
Wild allele (5'->3'): | GGACAGUACUUCAACGCUA
|
|
ASP-siRNA (As strand 3'->5'): | CCUGUCAUGUAGUUGCGAU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 30
|
|
Relative difference : | 50
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Fibroblast |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| Concentration used: | 10pmol |
| Reference: | 19289118 |
| Delivery method: | Transfection |
'| Article title: | A majority of Huntington's disease patients may be treatable by individualized allele-specific RNA interference. |
'| Authors: | Lombardi MS, Jaspers L, Spronkmans C, Gellera C, Taroni F, Di Maria E, Donato SD, Kaemmerer WF. |
'| Journal Reference: | Exp Neurol. 2009 Jun;217(2):312-9. doi: 10.1016/j.expneurol.2009.03.004. Epub 2009 Mar 13. |
'
