| | |
ASPsiRNA information |
siRNA Id: | aspsirna0060
|
siRNA Name: | si(T8/C9)
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | UUCACUUCUACCUACGUCUU
|
ASP-siRNA (As strand 3'->5'): | UUCACUUAGACCUACGUCUU
|
Mismatch position in siRNA: | A13C,G12U |
Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
Target Sequence (5'->3'): | AATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAAT
|
Wild allele (5'->3'): | AAGUGAAGAUGGAUGCAGAA
|
Mutant allele (5'->3'): | AAGUGAAUCUGGAUGCAGAA
|
Position of siRNA on target gene: | 2203-2222
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
Cytogenic location: | 21q21.3 |
Chromosomal coordinates: | 21:27,252,860-27,543,445 |
UniProt ID: | P56817 |
HUGO ID: | 620 |
Reference SNp(RefSNP): | rs28186516 |
Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
|
Matation type/variant: | Indel/missense variant
|
Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Alzheimers disease |
Clinical Resources |
ClinVar ID: | 18093 |
KEGG disease ID: | H00056 |
OMIM ID: | 104300 |
COSMIC: | APP |
DECIPHER: | APP |
GeneTests: | APP |
ASP siRNA details |
Mutant allele (5'->3'): | AAGUGAAUCUGGAUGCAGAA
|
ASP-siRNA (As strand 3'->5'): | UUCACUUAGACCUACGUCUU
|
Percentage efficacy of ASP-siRNA for mutant allele: | 20
|
Wild allele (5'->3'): | AAGUGAAGAUGGAUGCAGAA
|
ASP-siRNA (As strand 3'->5'): | UUCACUUAGACCUACGUCUU
|
Percentage efficacy of ASP-siRNA for wild allele: | 83
|
Relative difference: | -63
|
Wild siRNA details |
Wild allele (5'->3'): | AAGUGAAGAUGGAUGCAGAA
|
Wild siRNA (As strand 3'->5'): | UUCACUUCUACCUACGUCUU
|
Percentage efficacy of wild siRNA for wild allele: | 93
|
Wild allele (5'->3'): | AAGUGAAGAUGGAUGCAGAA
|
ASP-siRNA (As strand 3'->5'): | UUCACUUAGACCUACGUCUU
|
Percentage efficacy of Wild sirna for mutant allele: | 65
|
Relative difference : | 28
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HeLa |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Invitrogen |
Post-transfection duration: | 24 hours |
Concentration used: | 40nM |
Reference: | 19771217 |
Delivery method: | Transfection |
'Article title: | Assessment of allele-specific gene silencing by RNA interference with mutant and wild-type reporter alleles. |
'Authors: | Ohnishi Y, Tokunaga K, Kaneko K, Hohjoh H. |
'Journal Reference: | J RNAi Gene Silencing. 2006 Feb 28;2(1):154-60. |
'