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| ASPsiRNA information |
siRNA Id: | aspsirna0061
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siRNA Name: | si(T9/C10
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CUUCACUUCUACCUACGUCU
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ASP-siRNA (As strand 3'->5'): | CUUCACUUAGACCUACGUCU
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|
Mismatch position in siRNA: | A12C,G11U |
| Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
|
Target Sequence (5'->3'): | AATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAAT
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Wild allele (5'->3'): | GAAGUGAAGAUGGAUGCAGA
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Mutant allele (5'->3'): | GAAGUGAAUCUGGAUGCAGA
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Position of siRNA on target gene: | 2202-2221
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
| Cytogenic location: | 21q21.3 |
| Chromosomal coordinates: | 21:27,252,860-27,543,445 |
| UniProt ID: | P56817 |
| HUGO ID: | 620 |
|
Reference SNp(RefSNP): | rs28186516 |
| Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
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Matation type/variant: | Indel/missense variant
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Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
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Pathogenic status of mutation: | Pathogenic
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Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 18093 |
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KEGG disease ID: | H00056 |
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OMIM ID: | 104300 |
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COSMIC: | APP |
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DECIPHER: | APP |
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GeneTests: | APP |
| ASP siRNA details |
Mutant allele (5'->3'): | GAAGUGAAUCUGGAUGCAGA
|
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ASP-siRNA (As strand 3'->5'): | CUUCACUUAGACCUACGUCU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 42
|
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Wild allele (5'->3'): | GAAGUGAAGAUGGAUGCAGA
|
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ASP-siRNA (As strand 3'->5'): | CUUCACUUAGACCUACGUCU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 90
|
|
Relative difference: | -48
|
| Wild siRNA details |
Wild allele (5'->3'): | GAAGUGAAGAUGGAUGCAGA
|
|
Wild siRNA (As strand 3'->5'): | CUUCACUUCUACCUACGUCU
|
|
Percentage efficacy of wild siRNA for wild allele: | 93
|
|
Wild allele (5'->3'): | GAAGUGAAGAUGGAUGCAGA
|
|
ASP-siRNA (As strand 3'->5'): | CUUCACUUAGACCUACGUCU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 65
|
|
Relative difference : | 28
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| Concentration used: | 40nM |
| Reference: | 19771217 |
| Delivery method: | Transfection |
'| Article title: | Assessment of allele-specific gene silencing by RNA interference with mutant and wild-type reporter alleles. |
'| Authors: | Ohnishi Y, Tokunaga K, Kaneko K, Hohjoh H. |
'| Journal Reference: | J RNAi Gene Silencing. 2006 Feb 28;2(1):154-60. |
'
